Composite

Part:BBa_K3729005:Design

Designed by: Yi-Chen Lin   Group: iGEM21_KCIS_NewTaipei   (2021-10-17)


T7 promoter + Strong RBS + HBD gene + T7 terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1006
    Illegal XbaI site found at 1149
    Illegal PstI site found at 528
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1006
    Illegal NheI site found at 1045
    Illegal PstI site found at 528
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1006
    Illegal BamHI site found at 1012
    Illegal XhoI site found at 133
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1006
    Illegal XbaI site found at 1149
    Illegal PstI site found at 528
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1006
    Illegal XbaI site found at 1149
    Illegal PstI site found at 528
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

References